SOC Medium for Bacterial Transformation

SOC medium is a nutritionally rich ready-to-use broth useful in the final step of bacterial cell transformation to obtain maximal transformation efficiency of E. coli. Without magnesium chloride, needs to be added before use. Autoclave sterilized Applications SOC is a bacteriological growth medium used for the transformation of competent cells (E. coli). This nutrient-rich broth contains peptides, amino acids, water-soluble vitamins, and glucose in a low-salt formulation. More specifically SOC Medium can be used: in the propagation of DH-alpha (of E. coli strains) primarily in the recovery step of Escherichia coli competent cell transformations, and to maximize the transformation efficiency of competent cells. SOC medium formulation is SOB medium supplemented with 20mM glucose.   Recommendation Immediately before use, add 2M magnesium chloride to a final concentration of 20mM (10ml MgCl2/L).   Storage Conditions: 15°C to 30°C Shipping Condition: ambient temperature Autoclave Sterilized Max Shelf Life: 24 months from date of manufacture Harmonization Code: 3822.00.5090 UNSPSC: 41106200 ECCN: EAR99  

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Team:BGU Israel/Notebook1

Addgene: Protocol - Bacterial Transformation